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Innocent Meat

Serum Free Expansion Cell Media for Porcine Primary Muscle Cells

Serum Free Expansion Cell Media for Porcine Primary Muscle Cells

Early bird orders: get -50% off. Use promo code earlybirdIM

We start shipping 1st of October 2023

Regular price $377.00 USD
Regular price Sale price $377.00 USD
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Size
Pairs well with our porcine primary cells (muscle)
  • Serum-free FBS replacement
  • Suitable for primary and immortalized cells
  • High-performing, delivering reproducible results

 

We are unlocking the power of the bio-economy by ensuring the scalability of world-changing technologies such as regenerative medicine and cellular agriculture. Specifically designed for improved bioavailability and stability in 3D, large-scale cell culture applications.

Synonym: Proliferation Cell Media, Expansion Media, serum-free for porcine primary muscle cells.

Quantity: 500ml

Origin: The expansion media is produced in our laboratories in Rostock, Germany.

Species: porcine and bovine primary cells

Performance: high expansion rates

Safety: Currently for R&D Use only!

Contact us directly for large bulk industry production quantities.

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Performance

  • High cell expansion 

    A. Morphology of porcine satellite cells when cultured in Innocent Meat FBS-free (ADVSCEM04X) Porcine Satellite Cell Expansion Media across 96 hrs.

  • High Mygenin State

    B, C. Immunofluorescence images exhibiting the presence of key myogenic markers (PAX7 and Myf5) and differentiation markers such (Myogenin,MyoG) when grown in FBS-containing (B) and FBS-free (C) media.

  • Higher percentage of PAX7

    D. Percentage of PAX7 positive porcine satellite cells at Passage 05 when cultured in FBS-containing and FBS-free media.

    E. Percentage of Myf5 and MyoG positive porcine satellite cells at Passage 05 when cultured in FBS-containing and FBS-free media.

  • Proliferation assay

    F. Wst-1 based proliferation assay in porcine satellite cells when cultured in FBS-containing and FBS-free media.

  • Product descritpion and information

    Primary pig (porcine) muscle cells have been isolated from
    muscle tissue of pigs. Cell isolations were carried out with enzymatic digestion followed by culture in tissue culture flasks prior to cryopreservation. 


    Cells have been confirmed to proliferate for a minimum of 4 passages following the instructions provided and
    using recommended cell culture media.  Cells have tested negative for
    mycoplasma. 

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Animal Information

Species: Pig, Porcine, Sus scrofa

Tissue source: Muscle-derived

Vial and Storage Information

Vial properties: 1 mL Matrix cryovial

Product format: Frozen

Cell concentration: 500,000 cells in 0.5 mL

Storage conditions: Vapour phase liquid nitrogen

Mycoplasma: Not detected

Recommended Culture Conditions

Culture mode: Adherent

Growth conditions: 37 °C, 5% CO₂

Cell Media: Innocent Meat Expansion Media

Applications

For research use