Innocent Meat
Serum Free Expansion Cell Media for Porcine Primary Muscle Cells
Serum Free Expansion Cell Media for Porcine Primary Muscle Cells
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We start shipping 1st of October 2023
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- Serum-free FBS replacement
- Suitable for primary and immortalized cells
- High-performing, delivering reproducible results
We are unlocking the power of the bio-economy by ensuring the scalability of world-changing technologies such as regenerative medicine and cellular agriculture. Specifically designed for improved bioavailability and stability in 3D, large-scale cell culture applications.
Synonym: Proliferation Cell Media, Expansion Media, serum-free for porcine primary muscle cells.
Quantity: 500ml
Origin: The expansion media is produced in our laboratories in Rostock, Germany.
Species: porcine and bovine primary cells
Performance: high expansion rates
Safety: Currently for R&D Use only!
Contact us directly for large bulk industry production quantities.
Performance
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High cell expansion
A. Morphology of porcine satellite cells when cultured in Innocent Meat FBS-free (ADVSCEM04X) Porcine Satellite Cell Expansion Media across 96 hrs.
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High Mygenin State
B, C. Immunofluorescence images exhibiting the presence of key myogenic markers (PAX7 and Myf5) and differentiation markers such (Myogenin,MyoG) when grown in FBS-containing (B) and FBS-free (C) media.
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Higher percentage of PAX7
D. Percentage of PAX7 positive porcine satellite cells at Passage 05 when cultured in FBS-containing and FBS-free media.
E. Percentage of Myf5 and MyoG positive porcine satellite cells at Passage 05 when cultured in FBS-containing and FBS-free media.
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Proliferation assay
F. Wst-1 based proliferation assay in porcine satellite cells when cultured in FBS-containing and FBS-free media.
Porcine primary cells:
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Product descritpion and information
Primary pig (porcine) muscle cells have been isolated from
muscle tissue of pigs. Cell isolations were carried out with enzymatic digestion followed by culture in tissue culture flasks prior to cryopreservation.
Cells have been confirmed to proliferate for a minimum of 4 passages following the instructions provided and
using recommended cell culture media. Cells have tested negative for
mycoplasma.
Collapsible content
Animal Information
Species: Pig, Porcine, Sus scrofa
Tissue source: Muscle-derived
Vial and Storage Information
Vial properties: 1 mL Matrix cryovial
Product format: Frozen
Cell concentration: 500,000 cells in 0.5 mL
Storage conditions: Vapour phase liquid nitrogen
Mycoplasma: Not detected
Recommended Culture Conditions
Culture mode: Adherent
Growth conditions: 37 °C, 5% CO₂
Cell Media: Innocent Meat Expansion Media
Applications
For research use